Author(s)

Xingyu Chen ¹, Yulan Fan ², Xinyu Li ³

Affiliation(s)

¹ Cranleigh School, Horseshoe Ln, Cranleigh GU6 8QQ, UK
² RDF International School, Bai Zi Yuan Science and Technology Park, Kunan Road, Dapeng Street, Dapeng New District, Shenzhen City, Guangdong Province, China
³ Shenzhen Senior High School, No. 8, Zetian Road, Xiangmihu Street, Futian District, Shenzhen City, Guangdong Province, China

Corresponding Author

Xingyu Chen

ABSTRACT

Exosomes are nanosized phospholipid bilayer vesicles secreted by various cells, rich in bioactive molecules such as proteins, nucleic acids and lipids, which play important roles in intercellular communication and microenvironmental regulation. In recent years, T cell-derived exosomes have become a hotspot in tumor immunity research due to their immune specificity and functional diversity. The aim of this study was to systematically analyze the physicochemical properties of T cell-derived exosomes and their functional effects on tumor cells. T cell-derived exosomes were activated and isolated in vitro, and their particle size distribution, marker protein expression and cellular uptake ability were verified by nanoparticle tracking analysis, Western blot and confocal imaging. Functional experiments showed that T-cell exosomes can effectively inhibit tumor cell proliferation and induce apoptosis, suggesting that they have potential anti-tumor activity. This study provides experimental basis and theoretical support for the application of T-cell exosomes in tumor immunotherapy and expands its research horizons in the field of precision medicine.

KEYWORDS

T Cell, T Cell Exosome, Tumor Immunotherapy

CITE THIS PAPER

Xingyu Chen, Yulan Fan, Xinyu Li, Tumor-Infiltrating T Cells and Their Exosomes: Functional Roles and Microenvironmental Impact. Transactions on Cancer (2025) Vol. 6: 71-86. DOI: http://dx.doi.org/10.23977/tranc.2025.060110.

REFERENCES

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[3] Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007). Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nature cell biology, 9(6), 654–659. 
[4] Zhang, L., & Yu, D. (2019). Exosomes in cancer development, metastasis, and immunity. Biochimica et biophysica acta. Reviews on cancer, 1871(2), 455–468.
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[6] Tang, X. J., Sun, X. Y., Huang, K. M., Zhang, L., Yang, Z. S., Zou, D. D., Wang, B., Warnock, G. L., Dai, L. J., & Luo, J. (2015). Therapeutic potential of CAR-T cell-derived exosomes: a cell-free modality for targeted cancer therapy. Oncotarget, 6(42), 44179–44190.
[7] Li, L., Jay, S. M., Wang, Y., Wu, S. W., & Xiao, Z. (2017). IL-12 stimulates CTLs to secrete exosomes capable of activating bystander CD8+ T cells. Scientific reports, 7(1), 13365.

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