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High-Resolution Imaging and Automated Analysis for Veterinary Lateral Flow Immunoassays: Evaluation of the LF Reader Plus System

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DOI: 10.23977/medsc.2025.060310 | Downloads: 6 | Views: 179

Author(s)

Zhang Lei 1, Yang Feng 2, Zhu Junzhe 3

Affiliation(s)

1 Zhejiang Gongshang University, Hangzhou, Zhejiang, 310018, China
2 Community Health Service Center, Yipeng Street, Qiantang District, Hangzhou, Zhejiang, 310000, China
3 Wenzhou Medical University, Wenzhou, Zhejiang, 310000, China

Corresponding Author

Zhang Lei

ABSTRACT

The Lateral Flow (LF) Reader Plus integrates high-resolution imaging and automated analysis algorithms for veterinary lateral flow immunoassays (LFIAs). This study aims to evaluate its functional reliability and application scope through systematic testing on diverse animal specimens. Key functions (Setting, Records, Read, Upload, Print) and auxiliary features (self-test protocols, calibration routines) were validated. Operational workflows showed 100% reproducibility across 1200 tests. Compared with traditional devices, the reader demonstrates a 40% improvement in analysis speed and 35% enhancement in quantitative precision, supported by real-time thermal printing documentation. While assay-specific compatibility limitations exist, the device's clinical utility is underscored by its ability to deliver rapid, data-driven diagnostics. This study highlights its role as an efficient tool for veterinary point-of-care testing, advocating for expanded compatibility development and standardized user training.

KEYWORDS

Lateral Flow Reader Plus; Veterinary Diagnostics; Immunoassay; Automated Analysis

CITE THIS PAPER

Zhang Lei, Yang Feng, Zhu Junzhe, High-Resolution Imaging and Automated Analysis for Veterinary Lateral Flow Immunoassays: Evaluation of the LF Reader Plus System. MEDS Clinical Medicine (2025) Vol. 6: 68-73. DOI: http://dx.doi.org/10.23977/medsc.2025.060310.

REFERENCES

[1] Smith AB, Johnson CD. Advances in veterinary diagnostic technologies. Journal of Veterinary Science, 2019, 40(2), 123-135.
[2] Brown J, et al. Evaluation of a new lateral flow reader for veterinary diagnostics. Veterinary Record, 2023, 192(4), 89-95.
[3] Thompson D, et al. (2021). The role of veterinary diagnostics in global health security. Preventive Veterinary Medicine, 188, 105234.
[4] Wang Y, et al. Lateral flow immunoassays: Principles, applications, and future perspectives. Biosensors and Bioelectronics, 2020, 165, 112456.
[5] Yang H, et al. Improving the accuracy of lateral flow immunoassay through automated image analysis. Sensors, 2021, 21(10), 3456.
[6] Zhao Z, et al. Ultra-sensitive detection of biomarkers using lateral flow immunoassays with signal amplification. Biosensors and Bioelectronics, 2020, 160, 112134.
[7] Xu Y, et al. A review of signal amplification strategies for lateral flow immunoassays. Analytical Biochemistry, 2021, 625, 114256.
[8] Wu S, et al. Optimization of lateral flow immunoassay conditions for improved sensitivity and specificity. Sensors and Actuators B: Chemical, 2021, 330, 129345.

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