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  4. Vol 3, Issue 1, 2025

Construction of Exogenous Green Fluorescent Protein (GFP) Gene Expression Vector and Verification of Its Expression in Tobacco Leaves

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DOI: 10.23977/medbm.2025.030111 | Downloads: 8 | Views: 249

Author(s)

Zihou Hu 1

Affiliation(s)

1 Zihou Hu No.109 Senior High School, Dongcheng District, Beijing, China

Corresponding Author

Zihou Hu

ABSTRACT

This study aims to construct an exogenous green fluorescent protein (GFP) gene expression vector and verify its expression in tobacco leaves. First, the GFP gene was inserted into a plant expression vector containing a strong promoter to drive the expression of GFP by molecular cloning technology. Subsequently, the constructed vector was transformed into Escherichia coli for amplification, and the correctness of the inserted gene was verified by restriction endonuclease analysis and sequencing. Next, the recombinant vector was transferred into tobacco cells and infected by Agrobacterium-mediated transformation. The GFP expression in tobacco leaves was observed by fluorescence microscopy. The results showed that the transgenic tobacco plants emitted strong green fluorescence under ultraviolet light, proving that the GFP gene was successfully expressed. This study provides effective tools and methods for plant genetic engineering and lays a foundation for further research on plant cell biology.

KEYWORDS

Green Fluorescent Protein; Gene Cloning; Protein Expression; Agrobacterium-Mediated Transformation; Gene Expression

CITE THIS PAPER

Zihou Hu, Construction of Exogenous Green Fluorescent Protein (GFP) Gene Expression Vector and Verification of Its Expression in Tobacco Leaves. MEDS Basic Medicine (2025) Vol. 3: 65-77. DOI: http://dx.doi.org/10.23977/medbm.2025.030111.

REFERENCES

[1] Tang Hui, Lu Yao, Li Jinyan, Cheng Ke & Zhu Hongliang. (2024). Cultivation of tomatoes with visualized mitochondrial morphology based on green fluorescent protein(In Chinese). Journal of Biotechnology (04), 1029-1039. doi:10.13345/j.cjb.230488.
[2] Chen Mei, Chen Luqian, Chen Si, Du Changxia & Fan Huaifu. (2024). Optimization of Agrobacterium-mediated genetic transformation system of cucumber(In Chinese). Journal of Sichuan Agricultural University (03), 540-545+571. doi:10.16036/j.issn.1000-2650.202310365.
[3] Xia Jinhua, Li Dandan, Li Ran, Liu Xianni, Liu Zijie, Luo Bing & Ou Lijia. (2023). Cloning and functional analysis of the proliferation protein 2A-like gene of Huaiyushan three-leaf green tobacco virus(In Chinese). Jiangsu Agricultural Sciences (02), 36-43. doi:10.15889/j.issn.1002-1302.2023.02.005. 
[4] Yan Yaqin, Hu Tianhua, Wang Wuhong, Hu Haijiao, Wei Qingzhen, Wang Jinglei & Bao Chonglai. (2023). Establishment of the genetic transformation system of eggplant wilt pathogen mediated by Agrobacterium tumefaciens and construction of GFP-labeled strains(In Chinese). Journal of Agricultural Biotechnology (09), 1980-1988.
[5] Gao Chongtian, Chen Peng, Yuan Zhe & Sun Qun. (2009). Self-made serum antibody against green fluorescent protein (GFP) (In Chinese). Bulletin of Biology (08), 50-51.
[6] Gao Rui, Wu Huicai, Liu Chunju, Liu Zhongqing, Guan Ensen, Gao Qiang & Jiang Honghua. (2025). Research on tobacco plant detection and counting method based on improved YOLOv8(In Chinese). Journal of Chinese Tobacco Science (01), 111-122. doi:10.16472/j.chinatobacco.2024.T0153.
[7] Wang Peihan. (2020). Research progress on plasmid DNA extraction methods(In Chinese). Science and Technology Wind (02), 22. doi:10.19392/j.cnki.1671-7341.202002018.
[8] Feng Jie, Zeng Wendan, Yan Huabing, Wu Zhengdan & Wei Jie. (2024). Research progress on plant shoot apex regeneration and genetic transformation technology(In Chinese). Jiangsu Agricultural Science (13), 1-13. doi:10.15889/j.issn.1002-1302.2024.13.001
[9] Miao Hongping, Xia Liheng Bahatibek & Wang Xiaowei. (2024). Analysis on the current status and prospects of genetically modified soybeans in developing new quality productivity(In Chinese). Agricultural Outlook (11), 96-103.

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