Author(s)

Chen Zhoushun ¹, Tang Xiujun ¹

Affiliation(s)

¹ Zunyi Medical University, Zunyi, Guizhou, China

Corresponding Author

Chen Zhoushun

ABSTRACT

Keloid is a pathological fibrous proliferation disease characterized by tumor-like growth features. Its occurrence is closely related to the abnormal activation of fibroblasts and excessive deposition of the extracellular matrix. Due to the unclear pathogenesis, clinical treatment faces high recurrence rates, and there is a lack of safe and effective preventive and intervention methods. Single-Cell RNA Sequencing, scRNA-seq provides a new tool for revealing complex microenvironmental changes by systematically analyzing the transcriptional characteristics, heterogeneity, and interactions of various cells in Keloid tissues at the single-cell resolution. This review focuses on the application progress of scRNA-seq in Keloid research, systematically summarizing exploratory findings in characterizing key cell types such as fibroblasts, endothelial cells, Schwann cells, and immune cells, constructing cell communication networks, and screening potential therapeutic targets. The aim is to provide theoretical support for a deeper understanding of its pathogenesis and development of precise treatment strategies.

KEYWORDS

Single-cell transcriptome sequencing; keloid; fibroblast

CITE THIS PAPER

Chen Zhoushun, Tang Xiujun, Application and Perspectives of Single-Cell Transcriptomics in Unraveling the Pathogenic Mechanisms of Keloids. MEDS Basic Medicine (2025) Vol. 3: 55-64. DOI: http://dx.doi.org/10.23977/medbm.2025.030110.

REFERENCES

[1] Zhu Zhe. Epidemiological and Clinical Diagnosis and Treatment Status of Chinese Scar Inpatients [D]. PLA Navy Medical University, 2022. DOI:10.26998/d.cnki.gjuyu.2022.000157.
[2] Deng, C.C., et al., Single-cell RNA-seq reveals fibroblast heterogeneity and increased mesenchymal fibroblasts in human fibrotic skin diseases. Nat Commun, 2021. 12(1): p. 3709.
[3] Shim, J., et al., Integrated Analysis of Single-Cell and Spatial Transcriptomics in Keloids: Highlights on Fibrovascular Interactions in Keloid Pathogenesis. J Invest Dermatol, 2022. 142(8): p. 2128-2139.e11.
[4] Liu, X., et al., Single-Cell RNA-Sequencing Reveals Lineage-Specific Regulatory Changes of Fibroblasts and Vascular Endothelial Cells in Keloids. J Invest Dermatol, 2022. 142(1): p. 124-135 e11.
[5] Li, Q., et al., Single-cell transcriptome profiling reveals vascular endothelial cell heterogeneity in human skin. Theranostics, 2021. 11(13): p. 6461-6476.
[6] Liu, X., et al., Single-Cell RNA-Sequencing Reveals Lineage-Specific Regulatory Changes of Fibroblasts and Vascular Endothelial Cells in Keloids. J Invest Dermatol, 2022. 142(1): p. 124-135.e11.
[7] Direder, M., et al., Schwann cells contribute to keloid formation. Matrix Biol, 2022. 108: p. 55-76.
[8] Direder, M., et al., The transcriptional profile of keloidal Schwann cells. Exp Mol Med, 2022. 54(11): p. 1886-1900.
[9] Gong, T., et al., Single-cell RNA-seq reveals the communications between extracellular matrix-related components and Schwann cells contributing to the earlobe keloid formation. Front Med (Lausanne), 2022. 9: p. 1000324.
[10] Feng, C., et al., Single-cell RNA sequencing reveals distinct immunology profiles in human keloid. Front Immunol, 2022. 13: p. 940645.
[11] Shan, M., et al., The Role of CD28 and CD8(+) T Cells in Keloid Development. Int J Mol Sci, 2022. 23(16).
[12] Xu, H., et al., Downregulated cytotoxic CD8(+) T-cell identifies with the NKG2A-soluble HLA-E axis as a predictive biomarker and potential therapeutic target in keloids. Cell Mol Immunol, 2022. 19(4): p. 527-539.
[13] Wu, J., et al., RNA Sequencing Keloid Transcriptome Associates Keloids With Th2, Th1, Th17/Th22, and JAK3-Skewing. Front Immunol, 2020. 11: p. 597741.

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