Author(s)

Zhou Zicheng ¹

Affiliation(s)

¹ Vanke Meisha Academy, No. 33 Huan Mei Road, Dapeng New District, Shenzhen, Guangdong Province, China

Corresponding Author

Zhou Zicheng

ABSTRACT

Toxoplasma gondii is a prevalent parasite with global distribution capable of infection various warm-blooded animals, potentially causing clinical manifestations such as dirrhea and miscarriage. Investigation its protein expression through exerimental approached can enhance scientific understanding of this pathogen. This study mainly focuses on the epidemiological samples of T. gondii using the enzyme-linked immunosorbent assay (ELISA) to detect protein expression. Experimental procedures included PCR amplification. protein induction, expression, and purification, with recombinant protein validation performed through protein elextrophoresis. Results demonstrated that among 60 tested samples, a high proportion of false positives was observed, which did not correlate with the expected positivity rate (4 confirmed cases). This discrepancy may stem from sample contamination during non-standardized experimental procedures. These findings provide a reference for future T. gondii research and contribute to vaccine development efforts against this parasite. 

KEYWORDS

Toxoplasma gondii, ELISA, protein purification, PCR amplification, Toxoplasma gondii oocysts

CITE THIS PAPER

Zhou Zicheng, Expression of Toxoplasma Gondii Recombinant Protein and Its Application in ELISA Detection. MEDS Basic Medicine (2025) Vol. 3: 47-54. DOI: http://dx.doi.org/10.23977/medbm.2025.030109.

REFERENCES

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[2] Sheng, L., Xue, Q., Xu, S., Can, F., Yao, N., Zou, M., Teng, Q., Li, Y., El-Ashram, S., Ji, Y., & Zhao, J. (2023). Rapid and visual detection of Toxoplasma gondii oocyst in cat feces using loop-mediated isothermal amplification (LAMP) assay. Scientific Reports, 13(1). 
[3] Garibyan, Lilit, and Nidhi Avashia. "Polymerase chain reaction." The Journal of investigative dermatology vol. 133,3 (2013): 1-4. doi:10.1038/jid.2013.1
[4] Lu, Guili et al. "Prokaryotic expression of the V protein of the peste des petits ruminants virus and development of an indirect ELISA." Animal biotechnology vol. 34, 9 (2023): 5011-5015. doi:10.1080/10495398.2023.2221703

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