Author(s)

Jinhan Li ¹

Affiliation(s)

¹ Beijing Railway No.2 Middle School, Dianmen West Road, Xicheng District, Beijing, China

Corresponding Author

Jinhan Li

ABSTRACT

This study delved deeply into the effects of PS - 100 and NH₂ - PS - 100 on NCM460 cells, filling a void in the research regarding the impacts of these two specific microplastics on this type of cell within the realm of microplastic cytotoxicity. It laid the groundwork for the subsequent establishment of a more comprehensive theoretical framework of microplastic cytotoxicity. This study aimed to explore the effects of 100 - nanometer polystyrene microplastics (PS - 100) and 100 - nanometer amino - functionalized polystyrene microplastics (NH₂ - PS - 100) on normal human colorectal mucosal cells (NCM460 cells). NCM460 cells were treated with different concentrations of PS - 100, NH₂ - PS - 100, and their mixtures, and the cell viability was detected using the Cell Counting Kit - 8 (CCK - 8) assay. The results showed that the co - exposure of PS - 100 and NH₂ - PS - 100 could significantly reduce the viability of NCM460 cells in a concentration - and time - dependent manner. This finding provides a theoretical basis for understanding the potential health risks of microplastic.

KEYWORDS

Polystyrene microplastics; human colorectal mucosal cells; cytotoxicity CCK - 8

CITE THIS PAPER

Jinhan Li, Study on the Toxic Effects of Nanoplastics on Colonic Epithelial NCM460 Cells. MEDS Basic Medicine (2025) Vol. 3: 42-46. DOI: http://dx.doi.org/10.23977/medbm.2025.030108.

REFERENCES

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[3] Kumar R, Manna C, Padha S, Verma A, Sharma P, Dhar A, Ghosh A, Bhattacharya P. Micro(nano)plastics pollution and human health: How plastics can induce carcinogenesis to humans? Chemosphere. 2022 Jul;298:134267.
[4] Saha G, Chandrasekaran N. A comprehensive review on the adverse effect of microplastics in the gastrointestinal system of Artemia sp. Heliyon. 2024 Sep 11;10(18):e37720.   
[5] Bazeli J, Banikazemi Z, Hamblin MR, Sharafati Chaleshtori R. Could probiotics protect against human toxicity caused by polystyrene nanoplastics and microplastics? Front Nutr. 2023 Jul 10;10:1186724. 

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