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  4. Vol 4, Issue 2, 2024

Comparison of Quantitative Real-Time PCR and CLIA for the Detection of Hepatitis B Virus (HBV)

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DOI: 10.23977/phpm.2024.040214 | Downloads: 22 | Views: 917

Author(s)

Liang Ming 1, Zhaofan Luo 1

Affiliation(s)

1 Department of Clinical Laboratory, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, Guangdong, 518107, China

Corresponding Author

Zhaofan Luo

ABSTRACT

To investigate the value of quantitative real-time polymerase chain reaction (Real-Time PCR) and chemiluminescence method (CLIA) for the detection of hepatitis B virus (HBV). 321 HBV-DNA-positive patients from November to December 2023 were selected for the study objects, with the fasting blood collected from the patients for centrifugal serum separation. Then, Real-Time PCR and CLIA tests were performed to count the positive results of different methods and the copy number of Real-Time PCR. Among the 321 patients, there were 220 positive cases of HBsAg+, HBeAb+ and HBcAb+, the largest number that accounted for 68.32%. The mean values of different HBV-DNA loads varied, with the highest values for medium and high loads of HBsAg+, HBeAg+ and HBcAb+, and the highest values for low loads of HBsAb+, HBsAg+, HBeAb+ and HBcAb+. CLIA and Real-Time PCR correlate well with HBV laboratory tests, which can assist in the early diagnosis of hepatitis B and provide a favorable reference for the early treatment of the disease.

KEYWORDS

Hepatitis B virus; Quantitative Real-time polymerase chain reaction; Chemiluminescence method

CITE THIS PAPER

Liang Ming, Zhaofan Luo, Comparison of Quantitative Real-Time PCR and CLIA for the Detection of Hepatitis B Virus (HBV). MEDS Public Health and Preventive Medicine (2024) Vol. 4: 103-108. DOI: http://dx.doi.org/10.23977/phpm.2024.040214.

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